Both players were returned to Albany on Saturday, but the parent club was forced to summon the youngsters again due to a string of nagging injuries.
Forwards Chad LaRose, Scott Walker, Sergei Samsonov and Erik Cole all remain sidelined.
This marks the third call-up of the season for Boychuk, who picked up an assist in his first three games with Carolina this year.
Boychuk performed exceptionally well with the Canes this past Saturday against the New York Rangers. He was held scoreless, but chipped in defensively and was the driving force behind several offensive opportunities.
Tlusty also returns to Carolina for the third time this season. He has a goal and an assist in three games with the Canes this year. Tlusty earned the primary assist on the Canes' only regulation goal Saturday.
The call-up of Boychuk and Tlusty leaves the River Rats with a limited number of forwards.
Rob Hennigar is still out with an injury sustained in early December; Brad Herauf suffered a lower-body injury on Saturday; Michael Ryan has been out with an injury since last Wednesday; Nicolas Blanchard has one game remaining on a three-game suspension; and Matt Schepke was released Monday from a professional tryout contract.
The Rats will need reinforcements for tonight's contest against Adirondack if Ryan and Herauf are unable to play.